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Metoda > Techniki wykonania > genetic techniques (en) > PCR > amplified fragment length polymorphism (en)

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DEFINITION

  • AFLP is a PCR-based tool used in genetics research, DNA fingerprinting, and in the practice of genetic engineering. AFLP uses restriction enzymes to digest genomic DNA, followed by ligation of adaptors to the sticky ends of the restriction fragments. A subset of the restriction fragments is then selected to be amplified. (en)
  • Genetik çalışmalarda genomik DNA molekülünün restriksiyon enzimleri ile kesimi sonrası polimeraz zincir tepkimesi tekniği kullanılarak çoğaltılması ve elde edilen ürünün jel elektroforez bant modeline göre DNA üzerindeki polimorfizmin belirlenmesinde kullanılan yüksek duyarlılıktaki metot. (tr)

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URI

http://aims.fao.org/aos/agrovoc/c_331160

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